Phone: 406-243-2381
E-mail Address: Jesse.Hay@umontana.edu
Office : Skaggs 393
Personal Site: http://dbs.umt.edu/research_labs/haylabNearly every aspect of cell function depends upon the delivery of cellular products to their proper intracellular locations. Membrane-bound organelles receive their proteins, lipids and other constituents through inter-organelle exchanges mediated by ~50 nanometer spherical transport vesicles that bud from the donor compartment and are properly targeted to, and fuse with, the acceptor compartment. Transport vesicles contain special membrane proteins, called SNAREs, that are hypothesized to allow vesicles to dock and fuse at the appropriate acceptor membrane. Other proteins such as rabs, SM proteins and membrane tethers work with and regulate SNAREs to ensure accurate vesicle delivery and fusion. It is hard to imagine proteins more central to the organization and differentiation of intracellular membranes.
Dr. Hay's laboratory is trying to understand the molecular mechanism of action, regulation, and protein interactions of these vesicle trafficking proteins, using mammalian endoplasmic reticulum (ER)-to-Golgi and specialized neuronal membrane trafficking events as model systems. A wide variety of techniques are employed in this laboratory, including in vitro reconstitutions of transport processes in permeabilized cells, microscopy, and in vitro biochemistry.
PLEASE VISIT THE HAY LAB WEBSITE FOR MORE INFORMATION http://dbs.umt.edu/research_labs/haylab
Dalu Xu, Ashwini Joglekar, Antionette L. Williams, and Jesse C. Hay. 2000. Subunit Structure of a Mammalian ER/Golgi SNARE Complex. Journal of Biological Chemistry 275:39631-39639.
Jesse C. Hay. 2001. SNARE Complex Structure and Function. Experimental Cell Research 271:10-21.
Haruki Hasegawa, Sara Zinsser, Yeyoung Rhee, Einar Osland Vik-MO, Svend Davanger, and Jesse C. Hay. 2003. Mammalian Ykt6 is a Neuronal SNARE Targeted to a Specialized Compartment by its Profilin-like Amino Terminal Domain. Molecular Biology of the Cell 14:698-720.
Ashwini Joglekar, Dalu Xu, Daniel Rigotti, Robert Fairman, and Jesse C. Hay. 2003. The SNARE Motif Contributes to rbet1 Intracellular Targeting and Dynamics Independently of SNARE Interactions. Journal of Biological Chemistry 278:14121-14133.
Antionette L. Williams, Sebastian Ehm, Noëlle C. Jacobson, Dalu Xu and Jesse C. Hay. 2004. rsly1 Binding to Syntaxin 5 is Required for ER to Golgi Transport but Does Not Promote SNARE Motif Accessibility. Molecular Biology of the Cell 15: 162-175
Haruki Hasegawa, Zhifen Yang, Leif Oltedal, Svend Davanger and Jesse C. Hay. 2004. Intramolecular Protein-Protein and Protein-Lipid Interactions Control the Conformation and Subcellular Targeting of Neuronal Ykt6. J. Cell Science 117:4495-4508.
Dalu Xu and Jesse C. Hay. 2004. In Vitro Reconstitution of Homotypic COPII Vesicle Fusion to Generate a Pre-Golgi Intermediate Compartment. Journal of Cell biology 167:997-1003.
Ashwini Joglekar and Jesse C. Hay. 2005. Evidence for Regulation of ER/Golgi SNARE Complex Formation by hsp Chaperones in vitro. European Journal of Cell Biology 84:529-542.
Sidney Yu, Ayano Satoh, Marc Pypaert, Karl Mullen, Jesse C. Hay, and Susan Ferro-Novick. 2006. MBet3p is Required for Homotypic COPII Vesicle Tethering in Mammalian Cells. Journal of Cell Biology 174:359-368.
Marvin Bentley, Yingjian Liang, Karl Mullen, Dalu Xu, Elizabeth Sztul, and Jesse C. Hay. 2006. SNARE Status Regulates Tether Recruitment and Function in Homotypic COPII Vesicle Fusion. Journal of Biological Chemistry 281:38825-38833.
Huaqing Cai, Sidney Yu, Shekar Menon, Yiying Cai, Darina Lazarova, Chunmei Fu, Karin Reinisch, Jesse C. Hay, and Susan Ferro-Novick. 2007. TRAPPI Tethers COPII Vesicles by Binding the Coat Subunit Sec23. Nature 445:941-944.
Jesse C. Hay. 2007. Calcium: a Fundamental Regulator of Membrane Fusion? EMBO Reports 8:236-240.
